Beans SSR Markers

Beans:

  • Blair et. al 2013 designed primers for a total of 175 microsatellites with more than 20 bp of repeats identified with SSR locator from the collection of 21,000 ESTs from five different tissues of common bean: two types of leaves, nodules, pods and roots Table.
  • Wang et. al 2012 developed 13 microsatellites for P. vulgaris using the Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO) method.
  • Blair et. al. 2011 identified 184 microsatellites using 3,123 EST sequences from leaf and root cDNA libraries. Out of the 184 new SSR loci, 120 new microsatellite markers were developed in the BMc (Bean Microsatellites from cDNAs) series and evaluated for their capacity to distinguish bean diversity in a germplasm panel of 18 genotypes Table.
  • Kalavacharla et. al 2011 analyzed 59,295 454-derived sequences and 92,124 genomic sequences from common bean using MISA to detect 1,516 and 4,517 SSRs from 454-derived and genomic sequences respectively.
  • Córdoba et. al. 2010 carried out genome characterization study based on SSRs in 89,017 BAC end sequences (BESs) from the G19833 common bean (Phaseolus vulgaris L.) library. Together with 92 previously mapped BES- and 114 non-BES-derived markers, a total of 280 SSRs were included in the polymerase chain reaction (PCR)- based map, integrating a total of 8,232 BAC clones in 162 contigs from the physical map.
  • Hanai et. al. 2007 analyzed unigene collection of 3,126 sequences (generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/)) and a genomic microsatellite-enriched library for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs, whereas 714 genomic sequences contained 471 SSRs.
  • Yu et. al 2000 reported the first successful assignment of 15 SSR markers to the Phaseolus vulgaris molecular linkage map. A total of 37 SSR primer pairs were developed and tested for amplification and product-length polymorphism with BAT93 and Jalo EEP558, the parental lines of an F7 recombinant inbred (RI) population previously used for the construction of a common bean molecular linkage map.
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