Cowpea SSR Markers

Cowpea:

  • Gupta et. al. 2010 identified 1,071 SSRs from 15,740 cowpea unigene sequences downloaded from the National Center for Biotechnology Information. Primer pairs were successfully designed for 803 SSR motifs of which 102 SSR markers could be characterized and validated.
  • Xu et. al. 2010 developed 410 EST derived SSR (eSSR) markers and 600 SSR markers derived from cowpea genespace sequences (GSS) with a cross-subspecies transferability of 100% and 98.5%, respectively Table.
  • Xu P et. al. 2010 developed 1,372 SSR markers, including 494 EST-SSRs, 632 GSS-SSRs and 200 SSRs derived from BAC end sequences. Additional 46 SSR markers were developed by Li et. al. 2011.
  • Xu et. al 2007 analyzed representative set of 316 cowpea genetic resources from China, Africa and other countries of Asia. A total of 47 alleles were detected with an average of 3.692 alleles per SSR primer pair, and on an average 2.003 alleles from each SSR primer pair were effective. Thirteen reproducible and informative SSR primer pairs were selected from the 46 candidates.
  • Diaga Diouf et. al 2005 genetic diversity in local cowpea varieties and breeding lines from Senegal using random amplified polymorphic DNA (RAPD) and microsatellite (SSR) markers. Among the 61 RAPD primers used, twelve show polymorphism. in case of SSRs 15 of the 30 were polymorphic, detecting one to nine alleles per locus.
  • Cheng-Dao et. al. 2001 developed 46 SSRs to evaluate genetic similarities among 90 cowpea breeding lines. Twenty-seven primer pairs could amplify polymorphic single-locus microsatellites from all of these materials.

 

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