Efficient and fine mapping of RMES1 conferring resistance to sorghum aphid Melanaphis sacchari

Title 
Efficient and fine mapping of RMES1 conferring resistance to sorghum aphid Melanaphis sacchari 
Publication Type 
Journal Article 
Authors 
Wang F, Zhao S, Han Y, Shao Y, Dong Z, Gao Y, Zhang K, Liu X, Li D, Chang J, Wang D 
Year of Publication 
2013 
Volume 
31 
Journal 
Molecular Breeding 
Issue 
Pagination 
777 - 784 
Date Published 
4/2013 
ISSN 
1572-9788 
Keywords 
Aphid, BTx623, Fine mapping, Henong 16, HN16, Melanaphis sacchari, Molecular marker, RMES1, Sb6m2650, Sb6rj2776, Sorghum 
URL 
http://link.springer.com/article/10.1007/s11032-012-9832-6 
DOI 
10.1007/s11032-012-9832-6 
Abstract 

Melanaphis sacchari causes serious damage to sorghum (Sorghum bicolor (L.) Moench) growth, development and productivity in many countries. A dominant gene (RMES1) conferring resistance to M. sacchari has been found in the grain sorghum variety Henong 16 (HN16), but fine mapping of the RMES1 locus remains to be reported. In this study, genetic populations segregating for RMES1 were prepared with HN16 and BTx623 as parental lines. The latter had been used for sorghum genome sequencing but was found to be susceptible to M. sacchari in this work. A total of 11 molecular markers were mapped to the short arm of chromosome 6 harboring RMES1. The closest markers flanking the RMES1 locus were Sb6m2650 and Sb6rj2776, which delimited a chromosomal region of about 126 kb containing five predicted genes. The utility of the newly identified DNA markers for tagging RMES1 in molecular breeding of M. sacchari resistance, and further efforts in cloning RMES1, are discussed.

 
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