Evaluation of five peanut (Arachis hypogaea) genotypes to identify drought responsive mechanisms utilising candidate-gene approach

Title 
Evaluation of five peanut (Arachis hypogaea) genotypes to identify drought responsive mechanisms utilising candidate-gene approach 
Publication Type 
Journal Article 
Authors 
Dang PM, Chen CY, Holbrook CC 
Year of Publication 
2013 
Volume 
40 
Journal 
Functional Plant Biology 
Issue 
12 
Pagination 
1323 
Date Published 
09/2013 
ISSN 
1445-4408 
Keywords 
Abiotic stress, breeding, gene-expression, Groundnut 
URL 
http://www.publish.csiro.au/nid/102/paper/FP13116.htm 
DOI 
10.1071/FP13116 
Abstract 

Drought can significantly limit yield and quality in peanut (Arachis hypogaea L.), depending on its timing, duration and severity. The objective of this study was to identify potential molecular mechanism(s) utilising a candidate-gene approach in five peanut genotypes with contrasting drought responses. An early season drought stress treatment was applied under environmentally controlled rain-out shelters. When water was completely withheld for 3 weeks, no physical differences were observed for treated plants compared with their fully irrigated counterparts as indicated by relative water content; however, yield, grades (total sound mature kernel, TSMK), specific leaf area, and leaf dry matter content showed significant differences. Comparing expression levels of candidate genes, ‘C76–16’ exhibited significantly higher levels for CuZnSOD, NsLTP and drought protein 1 week earlier compared to the other genotypes, followed by significantly lower levels for the same genes. This suggested an early recognition of drought in C76–16 followed by an acclimation response. Cultivar ‘Georgia Green’ showed different patterns of gene-expression than C76–16. AP-3, a susceptible genotype, showed generally lower levels of gene-expression than C76–16 and Georgia Green. Myo-inositol phosphate synthase gene-expression showed high levels in irrigated treatment, ranging from 4-fold for 08T-12 to 12-fold for Georgia Green, but were significantly inhibited in drought treatment after 2 weeks of drought and after recovery.

 
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