Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers

Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers 
Publication Type 
Journal Article 
Ceylan A, Öcal N, Akbulut M 
Year of Publication 
Biochemical Systematics and Ecology 
219 - 229 
Date Published 
Beans, cpSSR, genetic diversity, Phaseolus vulgaris, POGP, SRAP 


  • Assessment of genetic diversity revealed considerable level of polymorphism in Turkish common bean cultivars.
  • Cluster analysis resulted in two main clusters possibly representing two major gene pools, namely Andean and Mesoamerican.
  • Small seeded cultivars separately clustered from others when either plastid or nuclear markers were used in UPGMA, NJ and PCA.

The genetic diversity among the Turkish cultivars of common bean (Phaseolus vulgaris L.) was estimated by studying the Sequence Related Amplified Polymorphism (SRAP), Peroxidase Gene Polymorhism (POGP), and Chloroplast Simple Sequence Repeats (cpSSR) markers. The unweighted pair group method arithmetic average (UPGMA) and Neighbor joining (NJ) algorithm resulted in a dendrogram representing the genetic relationship among major common bean cultivars grown in Turkey. The dendrogram generated two groups possibly representing two different major gene pools. By using three different marker systems, 194 alleles were detected and 118 were found to be polymorphic. For SRAP, POGP and cpSSR, 64, 64 and 26% polymorphism ratio were obtained, respectively. Principal Component Analysis (PCA) was also carried out to determine genetic variation among common bean genotypes and three different groups were generated. The individuals were placed into three different populations in structure analysis. Three populations created in structure analysis were exactly corresponded to the three groups in PCA. Analysis of Molecular Variance (AMOVA) was used to partition the genetic variations. The percentage of the variance was approximately 59%, 3%, and 38% among groups, among populations within groups and, within populations, respectively. The percentages of variation were found to be significantly high within the populations and among the groups.

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