Genetic enhancement of host plant-resistance of the Lalat cultivar of rice against bacterial blight employing marker-assisted selection

Title 
Genetic enhancement of host plant-resistance of the Lalat cultivar of rice against bacterial blight employing marker-assisted selection 
Publication Type 
Journal Article 
Authors 
Dokku P, Das KM, Rao GJN 
Year of Publication 
2013 
Volume 
35 
Journal 
Biotechnology Letters 
Issue 
Pagination 
1339 - 1348 
Date Published 
8/2013 
ISSN 
1573-6776 
URL 
http://link.springer.com/article/10.1007/s10529-013-1212-8 
Keywords 
bacterial blight, Gene pyramid, marker-assisted selection, Rice, Rice resistance 
DOI 
10.1007/s10529-013-1212-8 
Abstract 
To incorporate durable resistance against bacterial blight, a major disease rice, three resistance genes, xa 5, xa13 and Xa21, from IRBB 60 were transferred through marker-assisted backcrossing using RG 556, RG 136 and pTA248 markers linked to the three genes to supplement the Xa4 gene present in Lalat, a popular rice cultivar. Effective selection enabled the transfer in three back-crosses and a generation of selfing and background selection employing morphological and grain quality traits and molecular markers, led to >90 % recovery of the recurrent parental genome. The gene pyramids exhibited high levels of resistance against the pathogen in multi-location evaluation trials conducted over several locations of bacterial blight in India. IL-2 (CRMAS2621-7-1), a gene pyramid, was identified as being promising for several endemic regions of bacterial blight and was released as Improved Lalat in one of the identified regions. The success of the study demonstrates the vast potential of marker-assisted selection for gene stacking and recovery of the parental genome with high precision.
 
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