A High Throughput, Improved Rapid and Reliable Genomic DNA Extraction Protocol From Chickpea (Cicer arietinum L.)

Title 
A High Throughput, Improved Rapid and Reliable Genomic DNA Extraction Protocol From Chickpea (Cicer arietinum L.) 
Publication Type 
Journal Article 
Authors 
Kumar T, Bharadwaj C, Satyavathi TC, Jain PK 
Year of Publication 
2013 
Publisher 
Soceity of Plant Research 
Place Published 
India 
Journal 
VEGETOS 
Number 
26 
Issue 
Pagination 
185-190 
Date Published 
2013 
Keywords 
Chickpea, DNA extraction, Genogrinder, PCR assay 
DOI 
10.5958/j.2229-4473.26.2.073 
Abstract 

A high throughput, relatively rapid, reliable and inexpensive protocol for the genomic DNA isolation from chickpea is described. The procedure involved a modified cetyltrimethyl-ammonium bromide (CTAB) of and -mercaptoethanol buffer protocol with the use of indigenous genogrinder for DNA extraction from 100 mg of mature and young leaves of chickpea. The present protocol is also well suited for extracting high-molecular weight DNA from leguminous plant materials rich in polyphenols, tannins and polysaccharides. The DNA obtained by the present protocol is of very good quality, with no coloured pigment and contaminants, without using expensive liquid nitrogen and toxic phenols.

 
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