Identification and expression analysis of group 3 LEA family genes in sorghum [Sorghum bicolor (L.) Moench]

Title 
Identification and expression analysis of group 3 LEA family genes in sorghum [Sorghum bicolor (L.) Moench] 
Publication Type 
Journal Article 
Authors 
Dalal M, Sandeep Kumar G, Mayandi K 
Year of Publication 
2013 
Volume 
35 
Journal 
Acta Physiologiae Plantarum 
Issue 
Pagination 
979 - 984 
Date Published 
3/2013 
ISSN 
1861-1664 
Keywords 
Abiotic stress, Abscisic acid, Seed development, Sorghum 
URL 
http://link.springer.com/article/10.1007/s11738-012-1123-7 
DOI 
10.1007/s11738-012-1123-7 
Abstract 

Sorghum with its remarkable adaptability to drought and high temperature provides a model system for grass genomics and resource for gene discovery especially for abiotic stress tolerance. Group 3 LEA genes from barley and rice have been shown to play crucial role in abiotic stress tolerance. Here, we present a genome-wide analysis of LEA3 genes in sorghum. We identified four genes encoding LEA3 proteins in the sorghum genome and further classified them into LEA3A and LEA3B subgroups based on the conservation of LEA3 specific motifs. Further, expression pattern of these genes were analyzed in seeds during development and vegetative tissues under abiotic stresses. SbLEA3A group genes showed expression at early stage of seed development and increased significantly at maturity, while SbLEA3B group genes expressed only in matured seeds. Expression of SbLEA3 genes in response to abiotic stresses such as soil moisture deficit (drought), osmotic, salt, and temperature stresses, and exogenous ABA treatments was also studied in the leaves of 2-weeks-old seedlings. ABA and drought induced the expression of all LEA3 genes, while cold and heat stress induced none of them. Promoter analysis revealed the presence of multiple ABRE core cis-elements and a few low temperature response (LTRE)/drought responsive (DRE) cis-elements. This study suggests non-redundant function of LEA3 genes in seed development and stress tolerance in sorghum.

 
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