Interspecific chromosomal segment substitution line populations of Oryza sativa X O. glaberrima, O. merdionalis, and O. rufipogon

Source

GCP Project G3005.10 “Exploring Natural Genetic Variation: Developing Genomic Resources and Introgression Lines for Four AA Genome Rice Relatives”

Principal Investigator: Mathias Lorieux (IRD/CIAT) & Joe Tohme (CIAT), Cali, Colombia

Contact

Mathias Lorieux, CIAT, Cali, Colombia m.lorieux@cgiar.org

Product Description/Background

Wild relatives represent a valuable source of under-utilized genetic variation that is available to plant breeders and represent an invaluable source of genetic diversity for traits of interest. A systematic approach was used to develop three libraries of inter-specific lines called Chromosome Segment Substitution Lines (CSSLs), targeting chromosomal introgressions from different rice relatives and develop a set of  SNP markers identified in genes associated with tolerance to abiotic stress (drought, acid soils, mineral deficiencies or toxicities).

O. sativa X O. glaberrima:  A CSSL working population of 62 BC3DH interspecific lines bearing introgressed genome segments from the AA genome of the cultivated species, Oryza glaberrima,  in a O. sativa subspecies japonica background.

O. sativa X O. meridionalis: A CSSL working population of 32 BC3DH interspecific lines bearing introgressed genome segments from the AA genome of the wild species, Oryza meridionalis

O. sativa X O. rufipogon: A CSSL working population of  60 BC3DH  interspecific lines bearing introgressed genome segments from the AA genome of the wild species, Oryza rufipogon

Access/Availability

Available from the IRRI Gene Bank. 

Send a request either by:

1)  letter addressed to Dr. Nigel Ruaraidh Sackville Hamilton, Head, Genetic Resources

Center, IRRI, DAPO Box 7777 Metro Manila, Philippines; or

2) electronic mail to Dr. N. Ruaraidh Sackville Hamilton (r.hamilton@cgiar.org) or

3) access germplasm information directly thru the System-wide Information Network for

Genetic Resources (SINGER) website (http://www.cgiar.org/singer)

Supporting Publications

Bocco R, Lorieux M, Seck PA, Futakuchi K, Manneh B, Baimey H, Ndjiondjop MN (2012). Agro-morphological characterization of a population of introgression lines derived from crosses between IR 64 (Oryza sativa indica) and TOG 5681 (Oryza glaberrima) for drought tolerance. Plant Science 183:65–76, ISSN 0168-9452, (DOI: 10.1016/j.plantsci.2011.09.010). (G3005.10) Abstract

Garavito A, Guyot R, Lozano J, Gavory F, Samain S, Panaud O, Tohme T, Ghesquière A, and Lorieux M. (2010). A genetic model for the female sterility barrier between Asian and African cultivated rice species.Genetics 185:1425–1440  (DOI: 10.1534/genetics.110.116772). Abstract

Gutierrez A, Carabali S, Giraldo OX, Martinez CP, Correa F, Prado G, Tohme J and Lorieux M (2010). Identification of a rice Stripe Necrosis Virus resistance locus and yield component QTLs using Oryza sativa xO. glaberrima introgression lines. BMC Plant Biology 10:6.  PDF

Liakat Ali, M., P. Sanchez, Yu S, Lorieux M and Eizenga GC (2010). Chromosome Segment Substitution Lines: A Powerful Tool for the Introgression of Valuable Genes from Oryza Wild Species into Cultivated Rice (O. sativa). Rice 3 (4): 218–234. Abstract

Other Resources

PaddyMap:

Paddy Map is a database for easily generating sets of polymorphic SSR markers in any interspecific or intraspecific cross of rice (Oryza sativa L.). It is based on the concept of Universal Core Genetic Map that is being developing in the framework of a Generation Challenge Program project (see the publication Orjuela et al). 

Version 1 of the Universal Core Genetic Map for rice is referenced in Gramene as “CIAT SSR 2006”. 
Version 2, which contains several corrections for marker identities and locations, is available from the author and is included in Paddy Map. Contact Mathias Lorieux, IRD/CIAT, Cali, Colombia m.lorieux@cgiar.org

GCP Project Final Technical Report:

G3005.10 Exploring Natural Genetic Variation: Developing Genomic Resources and Introgression Lines for Four AA Genome Rice Relatives

 

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