Isozyme Diversity in Cassava Cultivars (Manihot esculenta Crantz)

Title 
Isozyme Diversity in Cassava Cultivars (Manihot esculenta Crantz)  
Publication Type 
Journal Article 
Authors 
Resende AG, Filho PSV, de Machado MFPS 
Year of Publication 
2000 
Publisher 
Springer 
Place Published 
New York 
Volume 
38 
Journal 
Biochemical Genetics 
Issue 
7/8 
Pagination 
203 - 216 
ISSN 
00062928 
Keywords 
acid phosphatase, Cassava, esterases, isozyme polymorphisms, malate dehydrogenases, Manihot esculenta., shikimate dehydrogenases 
URL 
http://www.springerlink.com/content/h2472706720n0680/ 
DOI 
10.1023/A:1001963319965 
Abstract 

Isoenzyme electrophoresis was used as a method to determine genetic diversity in various M. esculenta cultivars collected in the Southwestern (SW) and Northwestern (NW) regions of the State of Parana, in the South region of Brazil, and in cultivars produced at the Agronomic Institute of Campinas (IAC), S~ao Paulo State, Southeastern region of Brazil. The cultivars have been maintained by vegetative propagation for 5 years and are useful in production programs. A total of 28 loci in the acid phosphatase (ACP; EC 3.1.3.2), esterases (EST; EC 3.1.1.1), malate dehydrogenase (MDH; EC 1.1.1.37), and shikimate dehydrogenase (SKDH; EC 1.1.1.15) isozymes was analyzed. The proportion of polymorphic loci for NW, SW, and IAC cultivars was 57.14, 50.0, and 53.6%, respectively. Genetic diversity calculated by Nei''s genetic identity (I) showed high I values for the three M. esculenta subpopulations. The high degree of polymorphism expressed by cassava cultivars is highly relevant to stimulate breeding programs with M. esculenta species.

 
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