Marker-aided breeding for resistance to bean common mosaic virus in Kyrgyz bean cultivars

Title 
Marker-aided breeding for resistance to bean common mosaic virus in Kyrgyz bean cultivars 
Publication Type 
Journal Article 
Authors 
Hegay S, Ortiz R, Garkava-Gustavsson L, Hovmalm HP, Geleta M 
Year of Publication 
2013 
Journal 
Euphytica 
Date Published 
09/2013 
ISSN 
1573-5060 
URL 
http://link.springer.com/article/10.1007/s10681-013-0928-9 
Keywords 
Backcross breeding, Beans, DNA marker assisted selection, host plant resistance, Phaseolus vulgaris, Virus strains 
DOI 
10.1007/s10681-013-0928-9 
Abstract 

Common bean (Phaseolus vulgaris L.) is an important export crop in Kyrgyzstan. Bean common mosaic virus (BCMV) is a seed-born disease that affects this crop and reduces its yield significantly in the country. The aim of this study was to identify virus strain(s) occurring in Kyrgyzstan and breed host plant resistance to BCMV using DNA markers. Susceptible Kyrgyz cultivars (Ryabaya, Kytayanka and Lopatka) were included in a backcrossing breeding scheme for introducing host plant resistance from resistant cultivars (Vaillant and Flagrano). The virus strains were evaluated according to the symptoms of differential cultivars. The virus strain NL6 was found in northern Kyrgyzstan, where farmers grow most of the common bean produced in this country. Two SCAR markers (SW13 and SBD5) were used successfully in marker-aided backcrossing for pyramiding the I and bc-1 2 genes, which provide host plant resistance to BCMV. Resistant BC4F2 offspring carrying the I gene showed hypersensitivity reactions to necrosis inducing NL3 strain after detached leaf-assays.

 
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