Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea

Title 
Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea 
Publication Type 
Journal Article 
Authors 
Varshney RK, Mohan SM, Gaur PM, Chamarthi SK, Singh VK, Srinivasan S, Swapna N, Sharma M, Pande S, Varshney RK, Singh S, Kaur L 
Year of Publication 
2014 
Volume 
Journal 
The Plant Genome 
Issue 
Date Published 
03/2014 
Keywords 
ABQTL-I, ABQTL-II, Ascochyta blight, Background selection, BGS, C 214, Chickpea, Disease resistance, FGS, foc1 locus, foreground selection, Fusarium wilt, GA16, GAA47, ILC 3279, MABC, marker-assisted backcrossing, marker-assisted selection, MAS, molecular breeding, molecular markers, QTL, quantitative trait loci, recurrent parent genome, RPG, SCY17, simple sequence repeat, SSR, TA110, TA130, TA194, TA2, TAA60, TR19, TR58, TS82, WR 315 
URL 
https://www.crops.org/publications/tpg/abstracts/7/1/plantgenome2013.10.0035 
DOI 
10.3835/plantgenome2013.10.0035 
Abstract 

Fusarium wilt (FW) and Ascochyta blight (AB) are two major constraints to chickpea (Cicer arietinum L.) production. Therefore, two parallel marker-assisted backcrossing (MABC) programs by targeting foc1 locus and two quantitative trait loci (QTL) regions, ABQTL-I and ABQTL-II, were undertaken to introgress resistance to FW and AB, respectively, in C 214, an elite cultivar of chickpea. In the case of FW, foreground selection (FGS) was conducted with six markers (TR19, TA194, TAA60, GA16, TA110, and TS82) linked to foc1 in the cross C 214 × WR 315 (FW-resistant). On the other hand, eight markers (TA194, TR58, TS82, GA16, SCY17, TA130, TA2, and GAA47) linked with ABQTL-I and ABQTL-II were used in the case of AB by deploying C 214 × ILC 3279 (AB-resistant) cross. Background selection (BGS) in both crosses was employed with evenly distributed 40 (C 214 × WR 315) to 43 (C 214 × ILC 3279) SSR markers in the chickpea genome to select plant(s) with higher recurrent parent genome (RPG) recovery. By using three backcrosses and three rounds of selfing, 22 BC3F4 lines were generated for C 214 × WR 315 cross and 14 MABC lines for C 214 × ILC 3279 cross. Phenotyping of these lines has identified three resistant lines (with 92.7–95.2% RPG) to race 1 of FW, and seven resistant lines (with 81.7–85.40% RPG) to AB that may be tested for yield and other agronomic traits under multilocation trials for possible release and cultivation.

 
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