Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench)

Title 
Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench) 
Publication Type 
Journal Article 
Authors 
Gao J, Xia B, Luo F, Sun S, Pei Z, Gui Z, Yuan Q, Li X 
Year of Publication 
2013 
Volume 
193 
Journal 
Euphytica 
Issue 
Pagination 
383-390 
Date Published 
10/2013 
ISSN 
1573-5060 
URL 
http://link.springer.com/article/10.1007/s10681-013-0939-6 
Keywords 
BJ-299, BTx-622, CMS, Cytoplasmic-nuclear male sterility, Lunen-2, marker-assisted selection, MAS, rf1 gene, simple sequence repeat, Sorghum, sorghum A1 cytoplasm, SSR, Stalk juice content, Stalk juice sugar content, Xtxp18 
DOI 
10.1007/s10681-013-0939-6 
Abstract 

A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F5/BC3) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future.

 
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