Massive Sorghum Collection Genotyped with SSR Markers to Enhance Use of Global Genetic Resources

Title 
Massive Sorghum Collection Genotyped with SSR Markers to Enhance Use of Global Genetic Resources 
Publication Type 
Journal Article 
Authors 
Billot C, Ramu P, Bouchet S, Chantereau J, Deu M, Gardes L, Noyer J-L, Rami J-F, Rivallan R, Li Y, Lu P, Wang T, Folkertsma RT, Arnaud E, Upadhyaya HD, Glaszmann J-C, Hash TC 
Year of Publication 
2013 
Volume 
Journal 
PLoS ONE 
Issue 
Pagination 
e59714 
Date Published 
4/2013 
Keywords 
Bicolor, Caudatum, Durra, global sorghum germplasm collections, Guinea, marker-based groups, morphotypes, reference marker kit, reference nuclear SSR markers, Sorghum, weedy accessions, wild accessions 
URL 
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059714 
DOI 
10.1371/journal.pone.0059714.s005 
Abstract 

Large ex situ collections require approaches for sampling manageable amounts of germplasm for in-depth characterization and use. We present here a large diversity survey in sorghum with 3367 accessions and 41 reference nuclear SSR markers. Of 19 alleles on average per locus, the largest numbers of alleles were concentrated in central and eastern Africa. Cultivated sorghum appeared structured according to geographic regions and race within region. A total of 13 groups of variable size were distinguished. The peripheral groups in western Africa, southern Africa and eastern Asia were the most homogeneous and clearly differentiated. Except for Kafir, there was little correspondence between races and marker-based groups. Bicolor, Caudatum, Durra and Guinea types were each dispersed in three groups or more. Races should therefore better be referred to as morphotypes. Wild and weedy accessions were very diverse and scattered among cultivated samples, reinforcing the idea that large gene-flow exists between the different compartments. Our study provides an entry to global sorghum germplasm collections. Our reference marker kit can serve to aggregate additional studies and enhance international collaboration. We propose a core reference set in order to facilitate integrated phenotyping experiments towards refined functional understanding of sorghum diversity.

 
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