QUES, a new Phaseolus vulgaris genotype resistant to common bean weevils, contains the Arcelin-8 allele coding for new lectin-related variants

Title 
QUES, a new Phaseolus vulgaris genotype resistant to common bean weevils, contains the Arcelin-8 allele coding for new lectin-related variants 
Publication Type 
Journal Article 
Authors 
Zaugg I, Magni C, Panzeri D, Daminati MG, Bollini R, Benrey B, Bacher S, Sparvoli F 
Year of Publication 
2013 
Volume 
126 
Journal 
Theoretical and Applied Genetics 
Issue 
Pagination 
647 - 661 
Date Published 
3/2013 
ISSN 
1432-2242 
Keywords 
Acanthoscelides obtectus Say, APA proteins, Arc and arcelin-like polypeptides, Arc-4, Arc-8, arcelin, ARL, ARL-8, bean weevil, Beans, bruchid beetles, G12949 wild bean accession, Mexican bean weevil, phaseolin, phytohemagglutinin, QUES, Zabrotes subfasciatus Bohemian, α-amylase inhibitor 
DOI 
10.1007/s00122-012-2008-2 
Abstract 

In common bean (Phaseolus vulgaris L.), the most abundant seed proteins are the storage protein phaseolin and the family of closely related APA proteins (arcelin, phytohemagglutinin and α-amylase inhibitor). High variation in APA protein composition has been described and the presence of arcelin (Arc) has been associated with bean resistance against two bruchid beetles, the bean weevil (Acanthoscelides obtectus Say) and the Mexican bean weevil (Zabrotes subfasciatus Bohemian). So far, seven Arc variants have been identified, all in wild accessions, however, only those containing Arc-4 were reported to be resistant to both species. Although many efforts have been made, a successful breeding of this genetic trait into cultivated genotypes has not yet been achieved. Here, we describe a newly collected wild accession (named QUES) and demonstrate its resistance to both A. obtectus and Z. subfasciatus. Immunological and proteomic analyses of QUES seed protein composition indicated the presence of new Arc and arcelin-like (ARL) polypeptides of about 30 and 27 kDa, respectively. Sequencing of cDNAs coding for QUES APA proteins confirmed that this accession contains new APA variants, here referred to as Arc-8 and ARL-8. Moreover, bioinformatic analysis showed the two proteins are closely related to APA components present in the G12949 wild bean accession, which contains the Arc-4 variant. The presence of these new APA components, combined with the observations that they are poorly digested and remain very abundant in A. obtectus feces, so-called frass, suggest that the QUES APA locus is involved in the bruchid resistance. Moreover, molecular analysis indicated a lower complexity of the locus compared to that of G12949, suggesting that QUES should be considered a valuable source of resistance for further breeding purposes.

 
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