Regeneration and transformation of cassava

Title 
Regeneration and transformation of cassava  
Publication Type 
Journal Article 
Authors 
Raemakers CJJM, Sofiari E, Jacobsen E, Visser RGF 
Year of Publication 
1997 
Publisher 
Springer 
Place Published 
New York 
Volume 
96 
Journal 
Euphytica 
Issue 
Pagination 
153 - 161 
ISSN 
00142336 
URL 
http://www.springerlink.com/content/m378384j5w246163/ 
Keywords 
adventitious shoot formation, Cassava, cassava regeneration, review, somatic embryogenesis, transformation 
DOI 
10.1023/A:1002970925897 
Reprint Edition 
This revised version was published online in July 2006 with corrections to the Cover Date. 
Abstract 

A prerequisite for the development of a successful transformation system is the availability of efficient regeneration systems. Up to 1995 the only available regeneration system in cassava was an organized type of somatic embryogenesis. Transformation of these organized somatic embryogenic cultures with particle bombardment or Agrobacterium tumefaciens resulted in chimeric transformed embryos. However, the transformed sector was lost after repeated cycles of secondary somatic embryogenesis. After 1995 a less organized system of somatic embryogenesis was developed, so called friable embryogenic callus (FEC) and a system of adventitious shoot regeneration. The FEC regeneration system was combined successfully with particle bombardment. Selection of transgenic plants was based on either luciferase activity, or resistance to the aminoglycoside paromomycin or the herbicide phosphinothricin. Furthermore, protoplasts of FEC are able to regenerate into plants and can be transformed by electroporation. The adventitious shoot regeneration system was combined successfully with Agrobacterium tumefaciens. For this mature somatic embryos were cocultivated with Agrobacterium and cultured for adventitious shoot development. After selection based on the aminoglycoside geneticin or on hygromycin transgenic plants were formed.

 
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