A validated chickpea germplasm reference collection

Source

GCP Project G4005.01.04   Completing genotyping of the composite germplasm set of chickpea

Principal Investigator:  HD Upadhyaya, ICRISAT, Patancheru, India

Contact

Hari Upadhyaya, ICRISAT, Patancheru, India H.UPADHYAYA@cgiar.org

Product Description/Background

Worldwide, chickpea is the fourth largest grain legume crop, predominantly grown in Asia. CGIAR’s and NARS genebanks maintain large collections of chickpea germplasm. However, globally there has been very limited use of genebank materials for the genetic enhancement of chickpea due to the very large size of the collections and limited resources by researchers to evaluate accessions. To address this constraint, a composite collection of 3000 accessions was developed and profiled using 50 SSRs and high throughput assays.  A total of 1741 alleles were detected in the composite collection, of which 968 were rare and 773 common alleles.  A complete data set of 49 SSRs on 2915 accessions was used to dissect the structure and diversity in the composite collection and form this reference set of 300 accessions which captures 1315 (78%) of the 1683 composite collection alleles.  The reference set represents a critical resource for plant scientists to study new adaptive traits, discover new favorable alleles and also new parental lines for pre-breeding crosses.

Access/Availability

Access the following site for a detailed list:  http://www.icrisat.org/what-we-do/crops/ChickPea/Chickpea_Reference1.htm

For more information and ordering accessions of the reference set contact:

Dr H D Upadhyaya
Assistant Research Program Director-Grain Legumes 
and Principal Scientist and Head Gene Bank
ICRISAT-Patancheru 
Andhra Pradesh, India 
H.Upadhyaya@cgiar.org 

Supplementary Resources

Publications:

Hari D. Upadhyaya, Mahendar Thudi, Naresh Dronavalli, Neha Gujaria, Sube Singh, Shivali Sharma and Rajeev K. Varshney. Genomic tools and germplasm diversity for chickpea improvement. Plant Genetic Resources: Characterization and Utilization (2011) 9(1); 45–58.  doi:10.1017/S1479262110000468 http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8195837

Hari D Upadhyaya, Sangam L Dwivedi, Michael Baum, Rajeev K Varshney, Sripada M Udupa, Cholenahalli LL Gowda, David Hoisington and Sube Singh. Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.). BMC Plant Biology 2008, 8:106 doi:10.1186/1471-2229-8-106    http://www.biomedcentral.com/content/pdf/1471-2229-8-106.pdf

Associated SSR marker kit:

The reference kit results from the formation of the Chickpea Reference Set and is composed of 35 SSRs with a sequence list and protocol, germplasm checks, and allele sizes of markers for those checks.  Access the detailed list at   http://www.icrisat.org/Marker-Kits.html

Final Technical Report:

GCP Project G4005.01.04 Completing genotyping of the composite germplasm set of chickpea

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