A validated sorghum germplasm reference collection

Product Description/Background

Sorghum [Sorghum bicolor (L.) Moench] has an immense range of genetic variability available in Africa where domestication first occurred. Further diversity occurred in Asia due to the early introduction of the crop. Landraces and wild relatives of cultivated sorghum from these centers of diversity are rich sources of resistance to diseases, insect pests and other stresses such as high temperature and drought. They are also sources of traits to improve food and fodder quality, animal feed and industrial products. However, this natural genetic diversity is under threat due to the destruction of habitats, commercial agricultural practices, industrial and infrastructural activities, and large-scale adoption of improved cultivars. Collection and conservation of sorghum germplasm has been accelerated in the past four decades to prevent the extinction of landraces and wild relatives of cultivated sorghum. Since then, germplasm collection and conservation have become integral components of crop improvement programs at both national and international levels (Rosenow and Dahlberg 2000). However, globally there has been very limited use of genebank materials for the genetic enhancement of sorghum due to the very large size of the collections and limited resources by researchers to evaluate accessions. To address this constraint, a reference set of 375 accessions has been defined which captures 78% of the diversity (615 alleles) contained in the composite germplasm collection. The reference set represents a critical resource for plant scientists to study new adaptive traits, discover new favorable alleles and also new parental lines for pre-breeding crosses.

Access/Availability

Access the following site for a detailed list:  http://www.icrisat.org/crop-sorghum-genebank-reference.htm For more information and ordering accessions of the reference set contact:

Dr H D Upadhyaya
Assistant Research Program Director-Grain Legumes 
and Principal Scientist and Head Gene Bank
ICRISAT-Patancheru 
Andhra Pradesh, India 
H.Upadhyaya@cgiar.org 

Supplementary Resources

Associated SSR marker kit:

The reference kit results from the formation of the Sorghum Reference Set from a composite set of  3384 sorghum accessions and is composed of 48 SSRs with a sequence list, and allele sizes of markers.  Access the detailed list at http://sorghum.cirad.fr/SSR_kit/Publications

Also refer to the following for complete marker and background information: 

 Claire Billot, Ronan Rivallan, Mbaye Ndoye Sall, DanielL Fonceka, Monique Deu, Jean-Christophe Glaszmann, Jean-Louis Noyer, Jean-Francois Rami, Ange-Marie RisterrucciI, Patick Wincker, Puna Ramu and Charles T. Hash. A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae).  American Journal of Botany: e245–e250. 2012. http://oar.icrisat.org/5946/7/e245.full.pdf 

Publications:

Poster: Sarah McGrath, Jean-Francois Rami, Christophe Glaszmann. 2010. Genotyping validation of GCP reference sets

Billot C., Ramu P., Bouchet S., Chantereau J., Deu M. et al., 2013. Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources. PLoS One, 8: e59714. Doi:10.1371/journal.pone.005

Mutegi , E. , F. Sagnard , K. Semagn , M. Deu , M. Muraya , B. Kanyenji , S. De Villiers , Et Al. 2011 Genetic structure and relationships within and between cultivated and wild sorghum ( Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers. Theoretical and Applied Genetics 122 : 989 – 1004 . http://link.springer.com/article/10.1007%2Fs00122-010-1504-5?LI=true#page-1

Additional Resources 

Final Technical Report:  GCP Project G4005.01.03  Completing genotyping of the composite germplasm set of sorghum

Source

GCP Project G4005.01.04   Completing genotyping of the composite germplasm set of chickpea

Principal Investigator:  HD Upadhyaya, ICRISAT, Patancheru, India

Contact

Hari Upadhyaya, ICRISAT, Patancheru, India H.UPADHYAYA@cgiar.org

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